1/10/2023 0 Comments Mrna sequence![]() Sequencing technologies are becoming more accessible, allowing scientists to leverage large mRNA data sets to aid in clinical and diagnostic research. How are technologies for mRNA sequencing advancing? Luckily, there are several analysis software solutions on the market today that guide users and aid in interpretation. Once the samples have been sequenced, the scientist is then tasked with analysis, which can be cumbersome for new users. Because genes are differentially expressed, experimental design is another important consideration to ensure that all genes of interest are detectable and quantified properly, with scientists required to choose between sequencing the global gene expression profile of a sample or enriching certain genes to study them in isolation. Before mRNA can be used in a sequencing workflow, its quality must first be assessed using reliable, automated benchtop capillary electrophoresis systems. mRNA degrades significantly faster than DNA, so it is often frozen at -80 oC for long-term storage. MRNA sequencing has long been a core workflow in many labs however, it has a unique set of challenges. What are the challenges of analyzing mRNA? By conducting mRNA sequencing alongside DNA sequencing, some variants of unknown clinical significance can now be elucidated, improving diagnostic yield and aiding treatment. Most recently, just prior to the COVID-19 pandemic, mRNA was used to increase the clinical utility of diagnostic genetic tests (1). Now that high-resolution technologies such as next-generation sequencing are more accessible, mRNA is being considered at the individual sample level, allowing us to realize the benefits of personalized medicine. Insights from these experiments have allowed scientists to develop therapeutics to target faulty gene pathways that lead to disease. For this reason, scientists have used techniques such as quantitative real time-polymerase chain reaction (qRT-PCR) to understand how genes are regulated within a molecular pathway, or microarrays to understand how multiple genes are modulated in response to cellular changes. MRNA has been key to understanding molecular pathways that underlie disease susceptibility or drug response. What attention was on mRNA prior to COVID-19?
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